E. coli

Arg26-Glu177, Accession #P18510

Recombinant Human IL-1RA Protein, a monomer containing 152 amino acid residues, has a predicted molecular mass of approximately 17.1 kDa.

> 98% by SDS-PAGE gel and HPLC analyses.

Less than 0.1 EU/μg of rHuIL-1RA as determined by LAL test.

The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 10⁴ U/mg in the presence of 50 pg/ml rHuIL-1α.

Lyophilized from a 0.22 μm filtered solution in PBS. See CoA for details.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 1 month.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.