E. coli

Asn971-Arg1023, Accession #P01133

Recombinant Human EGF Protein, a monomer containing 53 amino acid residues, has a predicted molecular mass of approximately 6.2 kDa.

> 98% by SDS-PAGE gel and HPLC analyses.

Less than 0.1 EU/μg of rHuEGF as determined by LAL test.

The ED₅₀ was determined by a cell proliferation assay using balb/c 3T3 cells is 0.1 ng/ml, corresponding to a specific activity of 1 × 10^7 units/mg.

Lyophilized from a 0.22 μm filtered solution of in PBS. See CoA for details.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 1 month.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.