E. coli

Met; Phe27-Asn38, Accession #P01244, Ala37Val; Gly49-Ala118, Accession #P05019, Glu51Arg

Recombinant Human LR3 IGF-1 Protein, a monomer containing 83 amino acid residues, has a predicted molecular mass of approximately 9.1 kDa.

> 98 % by SDS-PAGE analyses.
> 95 % by RP-HPLC analyses.

Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL test.

Fully biologically active when compared to standard. The ED₅₀ as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ U/mg.

Lyophilized from a 0.22 μm filtered solution of in PB. See CoA for details.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 1 month.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.