CHO Cells

Ala279-Ser390, Accession #P01137

Recombinant Human TGF-beta 1 Protein, a disulfide-linked homodimer, each subunit containing 112 amino acid residues, has a predicted molecular mass of approximately 25.6 kDa.

> 98% by SDS-PAGE gel and HPLC analyses.

Less than 0.01 EU/μg of rHuTGF-β1 as determined by LAL test.

The ED₅₀ was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is 0.05 ng/ml, corresponding to a specific activity of 2 × 10⁷ units/mg.

Lyophilized from a 0.22 μm filtered solution in Acetonitrile and TFA. See CoA for details.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 1 month.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.