E. coli

Gln28-Cys232, Accession #Q13253

Recombinant Human Noggin Protein, a noncovalently-linked non-glycosylated homodimer, each subunit containing 205 amino acid residues, has a predicted molecular mass of approximately 46.1 kDa.

> 95% by SDS-PAGE gel and HPLC analyses.

Less than 0.1 EU/μg of rHuNoggin as determined by LAL test.

Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED₅₀ for this effect is 2.0-3.0 ng/ml of Noggin.

Lyophilized from a 0.22 μm filtered solution in acetonitrile, TFA. See CoA for details.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 1 month.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.