Recombinant Enhanced Green Fluorecence Protein

reGFP

价格：

询价

货号：

1O2401

数量：

加入询价单

在线咨询

技术参数

DESCRIPTION

Expression system	E. coli
Accession number	Met; Val; Ser2-Lys238, Accession #P42212, Phe64Leu, Ser65Thr, His231Leu
Amino acid sequence	MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
Molecular mass	Recombinant eGFP Protein, a non-glycosylated monomer containing 239 amino acid residues, has a predicted molecular mass of approximately 26.9 kDa.

SPECIFICATIONS

Purity	> 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin	Less than 1 EU/μg of reGFP as determined by LAL test.
Formulation	Lyophilized from a 0.22 μm filtered solution in PBS. See CoA for details.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

SHIPPING & STORAGE

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage

Please use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Lyophilized protein should be stored at ≤ -20°C, stable for 12 months after receipt.
Reconstituted protein solution can be stored at 2-8°C for 1 month.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

BACKGROUND

Enhanced Green Fluorescent Protein (eGFP) is an engineered variant of wild‑type GFP originally derived from Aequorea victoria. Compared with native GFP, eGFP contains two key amino acid mutations (F64L and S65T), which significantly improve folding efficiency, fluorescence intensity and photostability, and accelerate chromophore maturation at 37 °C. It exhibits strong green fluorescence with excitation at ~488 nm and emission at ~509 nm. As a widely used reporter and marker protein, recombinant eGFP is commonly expressed in E. coli with high purity. It can be applied in protein localization, molecular interaction studies, fluorescence calibration, positive control for expression system validation, and quality control of fluorescence‑related detection platforms.

产品中心